Course Information
Course title
 Molecular Biology 
Semester
 101-1 
Designated for
 VARIOUS PROGRAM  MOLECULAR MEDICINE PROGRAM  
Instructor
 LIN, JING-JER 
Curriculum Number
 md&ph5001 
Curriculum Identity Number
 420 U0100 
Class
  
Credits
 4 
Full/Half
Yr.
 Half 
Required/
Elective
 Required 
Time
 Tuesday 8,9(15:30~17:20) Thursday 8,9(15:30~17:20) 
Remarks
 The upper limit of the number of students: 200. 
Ceiba Web Server
 http://ceiba.ntu.edu.tw/1011molbio 
Course introduction video
 
Table of Core Capabilities and Curriculum Planning
 Association has not been established
Course Syllabus
Please respect the intellectual property rights of others and do not copy any of the course information without permission
Course Description

見醫衛共同課程時間表 

Course Objective
 讓研究基礎醫學的研究生，瞭解原核生物與真核細胞內，核酸與蛋白質
等巨分子的結構與功能，以奠定其醫學研究的基礎。
 
Course Requirement
  
Student Workload (expected study time outside of class per week)
  
Office Hours
  
Designated reading
  
References
  
Grading
    
Progress
Week
Date
Topic
第1週
 9/11,9/13  （I）Introduction
（II）DNA structure and function
(1)DNA as store of information
(A) Outline of major experiments that led to the acceptance of DNA as the
genetic material
(B) Structure of nucleic acid, base pairing, tautomeric shift, DNA-A,-B,-Z forms
(C) Denaturing and renaturing of DNA
(D) Supercoiled DNA and topoisomerase 
第2週
 9/18,9/20  (2) DNA technology, genomic size and genetic content
(3) Genome: organization and evolution 
第3週
 9/25,9/27  (4) Extrachromosome DNA, nucleosome, chromosome and chromatid
(5) DNA replication
(A) Prokaryotic system 
第4週
 10/02,10/04  (B) Eukaryotic system
(C) Telomere
(6) The dynamic genome
(A) Recombination and transposition of DNA in the prokaryotic genome
(B) Rearrangement and amplification in the eukaryotic genome 
第5週
 10/09,10/11  (7)DNA damage and mutation
(8)DNA repair and modification 
第6週
 10/16,10/18  第一次期中考 (考試範圍 9/11~10/11)
（III）Transcription, regulation and processing
(1) Prokaryotic Transcription and Eukaryotic Pol II Transcription 
第7週
 10/23,10/25  (2) Transcription and Epigenetic Regulation
(3) Eukaryotic transcription and regulation (eukaryotic RNA pol. I and III) 
第8週
 10/30,11/01  (4) Post-transcriptional processing, ribozyme and regulation （IV）Translation
(1) Assembly line for protein synthesis
(2) Transfer RNA is the translated adaptor 
第9週
 11/06,11/08  (3) Ribosomes provide a translation factory
(4) Codons and their significance
(5) Translation in cell free system
(6)未確定 
第10週
 11/13,11/15  (7) Post-translational modification
I. Structure
II.Protein ubiquitination
（V）Recombinant DNA technology（I）
 
第11週
 11/20,11/22  (1) Cloning of DNA：transformation and screening of transformants
(2) Construction of cDNA and genomic libraries
(3) Colony and plaque hybridization, Southern blot, Northern blot, Western blot analysis
(4) Primer extension, S1 nuclease mapping and RNase protection assay
(5) Differential display
(6) Site-directed mutagenesis and polymerase chain reaction
(7) Application of restriction fragment length polymorphism and variable number tandem repeat for genetic disease analysis
(8) Genome-wide sequencing strategies 
第12週
 11/27,11/29  (9) Phage strategies : control of lytic and lysogenic pathway
(10) Phage display library
(11) A brief introduction to animal viruses
第二次期中考(考試範圍 10/18~11/22)
（VI）Construction of the cells 
第13週
 12/04,12/06  (1) Signal transduction in prokaryotes
(2) Signal transduction in eukaryotes
張富雄
（VII）Genes in the development
(1) Immune diversity and immunotherapy 
第14週
 12/11,12/13  (2) Gene regulation and development
(3) Oncogenes
(4) Tumor suppressor genes 
第15週
 12/18,12/20  （VIII）Recombinant DNA technology（II）
(1) Expression of cloned gene in prokaryotic and eukaryotic cells
(2) Yeast genetics and its applications
(3) Applications of bioinformatics and proteomics in biomedical research 
第16週
 12/25,12/27  (4) Current development of genome projects and their impacts to human diseases
(5) Transgenic techniques 
第17週
 1/01,1/03  (6) Gene therapy
(A) Viral vectors
(B) Clinical Application
期末考 (考試範圍 12/04~01/03)